Oligosorbent

  • Aptamers = short single-stranded oligonucleotides (DNA or RNA, typically 20-100 base pairs) whose sequence is able to specifically bind (a) target molecule(s) with high affinities that can be equivalent to those of antibodies
    • Possibility to selectively trap a target analyte from a complex sample while removing other matrix components from final extract.
  • Aptamers are identified for each target molecule within randomly synthesized nucleic acid libraries by an iterative in vitro process of selection and amplification
    • Chemical synthesis
  • Affinities that can be equivalent to those of antibodies
    • Possibility to reach enrichment factors similar to those obtained with immunosorbents

Application of oligosorbents :
Analysis of Ochratoxin A in a wheat extract

  • Recent studies:
    • Development of oligosorbents (OS) for various molecules in various application fields
    • Evaluation of different types of solid sorbents allowing the grafting of aptamers
    • Miniaturization of oligosorbents by the grafting of aptamers on monoliths

Collaboration: ANR Mycodiag

Publications:

  • Aptamer-based sorbents for sample treatment – A review, , V. Pichon, et al, Anal. Bioanal Chem. 2014
  • Ochratoxin A / wheat extracts, W. Hadj Ali, et al , Anal Bioanal Chem 2014, 406, 1233-1240
  • Bioaffinity sorbent, V. Pichon, et al , Comprehensive Sampling and Sample Preparation, pp 359–388, 2012
  • Cocaine : post-mortem blood. B, Madru, et al , Talanta, 85 (2011) 616-624
  • Ochratoxin A / red wine, F. Chapuis-Hugon, et al, Anal. Bioanal. Chem 400 (2011) 1199-1207
  • cocaine / human plasma. Madru B et al ; Anal. Chem. 81 (2009) 7081-7086

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Practical information

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