Oligosorbent

• Aptamers = short single-stranded oligonucleotides (DNA or RNA, typically 20-100 base pairs) whose sequence is able to specifically bind (a) target molecule(s) with high affinities that can be equivalent to those of antibodies
  • Possibility to selectively trap a target analyte from a complex sample while removing other matrix components from final extract.
• Aptamers are identified for each target molecule within randomly synthesized nucleic acid libraries by an iterative in vitro process of selection and amplification
  • Chemical synthesis
• Affinities that can be equivalent to those of antibodies
  • Possibility to reach enrichment factors similar to those obtained with immunosorbents

Application of oligosorbents :
Analysis of Ochratoxin A in a wheat extract

• Recent studies:
  • Development of oligosorbents (OS) for various molecules in various application fields
  • Evaluation of different types of solid sorbents allowing the grafting of aptamers
  • Miniaturization of oligosorbents by the grafting of aptamers on monoliths

Collaboration: ANR Mycodiag

Publications:

• Aptamer-based sorbents for sample treatment – A review, , V. Pichon, et al, Anal. Bioanal Chem. 2014
• Ochratoxin A / wheat extracts, W. Hadj Ali, et al , Anal Bioanal Chem 2014, 406, 1233-1240
• Bioaffinity sorbent, V. Pichon, et al , Comprehensive Sampling and Sample Preparation, pp 359–388, 2012
• Cocaine : post-mortem blood. B, Madru, et al , Talanta, 85 (2011) 616-624
• Ochratoxin A / red wine, F. Chapuis-Hugon, et al, Anal. Bioanal. Chem 400 (2011) 1199-1207
• cocaine / human plasma. Madru B et al ; Anal. Chem. 81 (2009) 7081-7086