• Aptamers = short single-stranded oligonucleotides (DNA or RNA, typically 20-100 base pairs) whose sequence is able to specifically bind (a) target molecule(s) with high affinities that can be equivalent to those of antibodies
    • Possibility to selectively trap a target analyte from a complex sample while removing other matrix components from final extract.
  • Aptamers are identified for each target molecule within randomly synthesized nucleic acid libraries by an iterative in vitro process of selection and amplification
    • Chemical synthesis
  • Affinities that can be equivalent to those of antibodies
    • Possibility to reach enrichment factors similar to those obtained with immunosorbents

Application of oligosorbents :
Analysis of Ochratoxin A in a wheat extract

  • Recent studies:
    • Development of oligosorbents (OS) for various molecules in various application fields
    • Evaluation of different types of solid sorbents allowing the grafting of aptamers
    • Miniaturization of oligosorbents by the grafting of aptamers on monoliths

Collaboration: ANR Mycodiag


  • Aptamer-based sorbents for sample treatment – A review, , V. Pichon, et al, Anal. Bioanal Chem. 2014
  • Ochratoxin A / wheat extracts, W. Hadj Ali, et al , Anal Bioanal Chem 2014, 406, 1233-1240
  • Bioaffinity sorbent, V. Pichon, et al , Comprehensive Sampling and Sample Preparation, pp 359–388, 2012
  • Cocaine : post-mortem blood. B, Madru, et al , Talanta, 85 (2011) 616-624
  • Ochratoxin A / red wine, F. Chapuis-Hugon, et al, Anal. Bioanal. Chem 400 (2011) 1199-1207
  • cocaine / human plasma. Madru B et al ; Anal. Chem. 81 (2009) 7081-7086


See also...

Molecular recognition mechanism based stationary phase for trace analysis

For numerous application fields, the challenge relies on the necessity to detect compounds at the trace level (often sub µg/L). This last decade, (...) 

> More...

Multidimensional separations

Approach widely explored in gas chromatography (GCxGC) Innovating developments in the field of headspace sampling Demonstration of the (...) 

> More...


Practical information

Headmistress : Pr. Valérie Pichon
email : valerie.pichon (arobase) espci.fr
CBI’s Administrator : Isabelle Borsenberger +33 (0)1 40 79 46 35
Administrator Assitant : Hélène Dodier +33 (0)1 40 79 46 35