Laboratoire Sciences, Bioanalytiques et Miniaturisation : Miniaturization of selective sorbents and coupling with nanoLC-MS

Miniaturization of selective sorbents and coupling with nanoLC-MS

Synthesis and characterization of molecularly imprinted polymers (MIP) produced in situ as monolith in a 100 µm-diameter capillary and coupled on-line with nanoliquid chromatography

PhD : F. Bel Hadj, V. Thibert

Synthesis and characterization of immunosorbents and of oligosorbents by covalent grafting of the biomolecules (antibodies, aptamers) on monoliths prepared with organosilanes ( obtained by Sol-Gel approach) and coupled on-line with nanoliquid chromatography

PhD students : F. Brothier (2011-2014), E. Fazel (2014-2016)

Publications :

Aptamer-based sorbents for sample treatment – A review, V. Pichon, et al, Anal. Bioanal Chem. 2014
Miniaturisation de supports à base d’anticorps et d’aptamères pour l’analyse de traces, F. Brothier, et al, L’actualité chimique – nov 2014 - n° 390
Immobilized antibody on a hybrid organic-inorganic monolith: Capillary immunoextraction coupled on-line to nanoLC-UV for the analysis of microcystin-LR, F. Brothier; et al Anal Chim Acta 2013,792, 52-58
Bioaffinity sorbent, V. Pichon et al , In Comprehensive Sampling and Sample Preparation, Elsevier, Academic Press: Oxford, pp 359–388, 2012

– Goal : Developing high performance and mass production compatible columns taking advantage of the specificity offered by the columns on chip (...)

Transfer to chip format of new electrokinetic methods developped first in classical capillary format to save time and money, such as an example (...)

The laboratory